Effect of endurance exercise on the Ca pumps from transverse tubule and sarcoplasmic reticulum of rabbit skeletal muscle
نویسندگان
چکیده
Viola Becker, Hugo González-Serratos, Rocı́o Álvarez, Michael Bäermann, Claudine Irles, and Alicia Ortega Departamento de Bioquı́mica, Facultad de Medicina, Universidad Nacional Autónoma de Mexico, México City 04510, México; Department of Physiology, School of Medicine, University of Maryland, Baltimore, Maryland 21201; Department of Physics E22 (Biophysics), Technical University of Munich, 80333 Garching, Germany; and Departamento de Bioquı́mica, Instituto Nacional de Perinatologı́a, México City 11000, México
منابع مشابه
Effect of endurance exercise on the Ca2+ pumps from transverse tubule and sarcoplasmic reticulum of rabbit skeletal muscle.
The sarcoplasmic reticulum (SR) Ca(2+) pump is the main homeostatic regulatory mechanism in fast skeletal muscle that maintains intracellular Ca(2+) concentration ([Ca(2+)](i)) at the nanomolar level at rest. The transverse tubule (TT) Ca(2+) pump transports cytosolic Ca(2+) to the extracellular space. During prolonged muscular activity, [Ca(2+)](i) may increase. TT and SR isolated microsomal v...
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In vertebrate skeletal muscle, the voltage-dependent mechanism of rapid sarcoplasmic reticulum (SR) Ca2+ release, commonly referred to as excitation-contraction (EC) coupling, is believed to be mediated by physical interaction between the transverse (T)-tubule voltage-sensing dihydropyridine receptor (DHPR) and the SR ryanodine receptor (RyR)/Ca2+ release channel. In this study, differential T-...
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A procedure has been devised for isolation of triads (t-tubule/sarcoplasmic reticulum (SR) junctional complexes) from rabbit skeletal muscle. The procedure consists of preparation of a heavy microsomal fraction followed by two sequential 90-min sucrose gradient centrifugations to enrich the triads. A pyrophosphate/phosphate/magnesium buffer system was introduced to decrease aggregation in order...
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Despite multiple procedures used to isolate transverse tubule vesicles from rabbit skeletal muscle, few proteins have been identified and shown to be specific to transverse tubule vesicles. Markers for purified transverse tubules have included high affinity dihydropyridine binding, cholesterol content, Mg2+-ATPase activity, (Na+,K+)-ATPase activity, and [3H] ouabain binding. Despite these marke...
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Isometric force and "Ca efflux from the sarcoplasmic reticulum were measured at 19°C in frog skeletal muscle fibers skinned by microdissection . After Ca" loading, application of the ionophores monensin, an Na+(K+)/ H' exchanger, or gramicidin D, an H+ > K' > Na' channel-former, evoked rapid force development and stimulated release of ^"30% of the accumulated "Ca within 1 min, whereas CCCP (car...
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تاریخ انتشار 2004